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Sci Rep:DNA快速分离术让基因检测更快捷
作者: 来源:半岛晨报 2014-07-29

   导读:近日,日本名古屋大学研究人员日前宣布,他们开发出了只需数秒就能分离脱氧核糖核酸(DNA)的新技术,这让超高速解析DNA信息成为可能,有望实现即时基因检测。

  

 

  日本名古屋大学研究人员日前宣布,他们开发出了只需数秒就能分离脱氧核糖核酸(DNA)的新技术,这让超高速解析DNA信息成为可能,有望实现即时基因检测。

  DNA是承载着生命信息的物质,在细胞内以双螺旋链的形式存在,对其进行分离、解析是一件非常繁琐耗时的工作。分离DNA需要根据DNA链的长短使用不同的材料,这使得现有的dna分离、解析技术最短需要数十个小时。

  名古屋大学的一份新闻公报称,该校研究人员开发出的新技术被称为“纳米圣诞树”――在一个长2厘米、宽1厘米、厚1毫米的特殊玻璃板上,像树枝一样布设大量的氧化锡纳米材料。当采集的血样在玻璃板上流动时,纳米材料“树枝”的缝隙会捕获不同长短的DNA链,数秒内就能分离出不同的DNA。

  快速分离DNA可以让超高速基因检测成为可能,而基因检测所得的信息将在疾病诊断和预防中发挥重要作用。这一成果已发表在《科学报告》杂志上,该研究团队并为此申请了技术专利。

  参考文献 

       Ultrafast and Wide Range Analysis of DNA Molecules Using Rigid Network Structure of Solid Nanowires

  文献检索:doi:10.1038/srep05252

  Analyzing sizes of DNA via electrophoresis using a gel has played an important role in the recent, rapid progress of biology and biotechnology. Although analyzing DNA over a wide range of sizes in a short time is desired, no existing electrophoresis methods have been able to fully satisfy these two requirements. Here we propose a novel method using a rigid 3D network structure composed of solid nanowires within a microchannel. This rigid network structure enables analysis of DNA under applied DC electric fields for a large DNA size range (100 bp–166 kbp) within 13 s, which are much wider and faster conditions than those of any existing methods. The network density is readily varied for the targeted DNA size range by tailoring the number of cycles of the nanowire growth only at the desired spatial position within the microchannel. The rigid dense 3D network structure with spatial density control plays an important role in determining the capability for analyzing DNA. Since the present method allows the spatial location and density of the nanostructure within the microchannels to be defined, this unique controllability offers a new strategy to develop an analytical method not only for DNA but also for other biological molecules.